Trypanosoma cruzi has nutritional requirements for essential metabolites, where heme group other cofactors such as iron, copper, calcium, etc. metal ions are included. This parasite lacks a route for heme synthesis, then it must guarantee the acquisition, storage, and correct use of heme throughout its life cycle. This characteristic is shared by other trypanosomatids responsible for diseases in humans such as T. brucei, Leishmania spp., and other parasites. In our laboratory, we study the molecular mechanisms developed by T. cruzi that govern heme homeostasis. The identification of transport proteins and/or chaperones that participate in these processes, and their subsequent functional and structural characterization, will generate relevant information on essential processes for the proliferation and survival of the parasite. This knowledge will validate this pathway as a possible chemotherapeutic target for the design of drugs for new treatments against Chagas disease.