research groups

Microorganisms of Agronomical and Environmental Interest

summary

In our group basic and applied microbiology research lines are carried out. In terms of basic research, environmental contaminant degradation pathways are studied, as well as pathways regulating the formation of bacterial biofilms and their relationship with metal oxidation. Regarding applied microbiology, the group works following premises tending to solve agricultural and environmental problems. In this context, pathogenic bacteria that cause plant diseases, beneficial bacteria capable of promoting plant growth and bioremediating contaminants present in the environment such as metals and herbicides are studied.
GROUP DIRECTOR
Ottado, Jorgelina

Sede:

CCT

Email:

ottado@ibr-conicet.gov.ar
Project Directors
  • Garavaglia, Betiana Soledad
Associated researchers
PhD POST-Fellows
PhD Fellows
  • Di Paolo, Valeria
  • Escalante, Julián
  • Lencina, Nicolás Martín
  • Muñoz, Ana Ines
Undergraduate Students
technicians

RESEARCH LINES

Bioremediation of glyphosate herbicide

The practice of direct sowing implies an increase in the use of agrochemicals; among them, the broad-spectrum foliar herbicide, glyphosate. This herbicide acts on the shikimate pathway of plants through the inhibition of the enzyme 5- enolpyruvylshikimate 3-phosphate synthetase (EPSPS), involved in the metabolism of aromatic amino acids. Glyphosate has become the most widely used herbicide in the world due to the introduction of GM crops resistant to it. The World Health Organization has classified it within group 2A, which includes possible carcinogens for humans. The excessive and inappropriate use of glyphosate is associated with its widespread presence in aquatic and terrestrial environments. Therefore, it is essential to have systematic controls of concentrations of the herbicide and its degradation compounds. For this, the development of tools that allow the detection and detoxification of the glyphosate molecule is proposed. In our group, bacteria obtained from environments contaminated with glyphosate have been isolated, identified and sequenced, including the strain Agrobacterium tumefaciens CHLDO, which has proven to be efficient to degrade glyphosate added to the culture medium. The general objective of this project is to characterize this degradation pathway, including its regulation, and to design biotechnological tools that enable the detection and removal of glyphosate from the environment.

Images of our research lines

PUBLICATIONS AND PATENTS

Bacterial isolates from Argentine Pampas and their ability to degrade glyphosate.

Sci Total Environ. 2021; 774:145761. Masotti F, Garavaglia BS, Piazza A, Burdisso P, Altabe S, Gottig N, Ottado J.

Bacterial isolates from Argentine Pampas and their ability to degrade glyphosate.

Sci Total Environ. 2021; 774:145761. Masotti F, Garavaglia BS, Piazza A, Burdisso P, Altabe S, Gottig N, Ottado J.
DOI

A novel Xanthomonas citri subsp. citri NADPH quinone reductase involved in salt stress response and virulence.

Biochim Biophys Acta Gen Subj. 2020; 1864(3):129514. Barcarolo MV, Garavaglia BS, Gottig N, Ceccarelli EA, Catalano-Dupuy DL, Ottado J.

A novel Xanthomonas citri subsp. citri NADPH quinone reductase involved in salt stress response and virulence.

Biochim Biophys Acta Gen Subj. 2020; 1864(3):129514. Barcarolo MV, Garavaglia BS, Gottig N, Ceccarelli EA, Catalano-Dupuy DL, Ottado J.
DOI

Participation of two general stress response proteins from Xanthomonas citri subsp. citri in environmental stress adaptation and virulence.

FEMS Microbiol Ecol. 2020; 96(8): fiaa138. Barcarolo MV, Gottig N, Ottado J, Garavaglia BS.

Participation of two general stress response proteins from Xanthomonas citri subsp. citri in environmental stress adaptation and virulence.

FEMS Microbiol Ecol. 2020; 96(8): fiaa138. Barcarolo MV, Gottig N, Ottado J, Garavaglia BS.
DOI

Optimization of bacterial bioaugmentation for groundwater Mn removal using a waste-based culture medium and lyophilization.

Environ. Sci.: Water Res. Technol. 2020; 6, 3255-3261. Ciancio Casalini L, Vidoz M, Piazza A, Labanca C, Pacini VA, Ottado J, Gottig N.

Optimization of bacterial bioaugmentation for groundwater Mn removal using a waste-based culture medium and lyophilization.

Environ. Sci.: Water Res. Technol. 2020; 6, 3255-3261. Ciancio Casalini L, Vidoz M, Piazza A, Labanca C, Pacini VA, Ottado J, Gottig N.
DOI

Environmental bacteria involved in manganese(II) oxidation and removal from groundwater.

Front Microbiol. 2019; 10:119. Piazza A, Ciancio Casalini L, Pacini VA, Sanguinetti G, Ottado J, Gottig N.

Environmental bacteria involved in manganese(II) oxidation and removal from groundwater.

Front Microbiol. 2019; 10:119. Piazza A, Ciancio Casalini L, Pacini VA, Sanguinetti G, Ottado J, Gottig N.
DOI

contacto@conicet.gov.ar

Sede CCT Rosario

Ocampo y Esmeralda, Predio CONICET-Rosario
2000 Rosario, Santa Fe, Argentina
Tel. 54-341-4237070 / 4237500 / 4237200

Sede Facultad de Ciencias Bioquímicas y Farmacéuticas

Universidad Nacional de Rosario - Suipacha 531
2000 Rosario, Santa Fe, Argentina
Tel. +54 341 4350596 / 4350661 / 4351235

✨Retreat IBR 2024: Comunidad y Motivación

Ayer, más de 140 integrantes del IBR compartimos una jornada única en el @ComplejoCAM

Una experiencia que nos inspira a seguir transformando juntos.

Gracias al equipo del @ComplejoCAM por su generosidad y atención impecable. 👏